1 - Wavefront sensing for aberration modeling in fluorescence MACROscopy.
P. Pankajakshan and A. Dieterlen and G. Engler and Z. Kam and L. Blanc-Féraud and J. Zerubia and J.C. Olivo-Marin. In Proc. IEEE International Symposium on Biomedical Imaging (ISBI), Chicago, USA, April 2011.
Keywords : fluorescence MACROscopy , phase retrieval, field aberration.
@INPROCEEDINGS{PanjakshanISBI2011,
|
| author |
= |
{Pankajakshan, P. and Dieterlen, A. and Engler, G. and Kam, Z. and Blanc-Féraud, L. and Zerubia, J. and Olivo-Marin, J.C.}, |
| title |
= |
{Wavefront sensing for aberration modeling in fluorescence MACROscopy}, |
| year |
= |
{2011}, |
| month |
= |
{April}, |
| booktitle |
= |
{Proc. IEEE International Symposium on Biomedical Imaging (ISBI)}, |
| address |
= |
{Chicago, USA}, |
| url |
= |
{http://hal.inria.fr/inria-00563988/en/}, |
| keyword |
= |
{fluorescence MACROscopy , phase retrieval, field aberration} |
| } |
Abstract :
In this paper, we present an approach to calculate the wavefront in
the back pupil plane of an objective in a fluorescent MACROscope.
We use the three-dimensional image of a fluorescent bead because it
contains potential pupil information in the ‘far’ out-of-focus planes
for sensing the wavefront at the back focal plane of the objective.
Wavefront sensing by phase retrieval technique is needed for several
reasons. Firstly, the point-spread function of the imaging system
can be calculated from the estimated pupil phase and used for image
restoration. Secondly, the aberrations in the optics of the objective
can be determined by studying this phase. Finally, the estimated
wavefront can be used to correct the aberrated optical path with-
out a wavefront sensor. In this paper, we estimate the wavefront of
a MACROscope optical system by using Bayesian inferencing and
derive the Gerchberg-Saxton algorithm as a special case. |
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